The overall scientific objective of the project is to develop from concept a novel type of medical diagnostic devices able to provide reliable clinical measurements at resource-limited or poor settings, where centralized laboratory facilities are in short supply, and for use at home. The proposed diagnostic devices employing novel responsive polymer-based biosensors and their operation is based on newly devised transduction and measuring principles. Diagnostic devices will be tested for the determination of urea, creatinine and uric acid, which are employed as model biochemical indexes, in biological fluids (blood and urine), as well as, for the detection of Helicobacter pylori (H. Pylori) in bioptic samples and exhaled breath or exhaled breath condensate. The proposed diagnostic devices can be used as a generic platform for the point-of-care analysis of a large variety of biochemical indices, in accordance with the “ASSURED” criteria set by the world health organization (WHO). Wide scope applicability can be achieved by the proper combination of responsive polymer membranes and enzymes that will be immobilized onto the surface of the membranes. Medical diagnostic devices will be designed to have small size, light weight, low power consumption and they will be developed in two different types: i) as a stand-alone portable device and b) as a compact “diagnostic card” with wireless communication and smartphone-aided operation. There are six types of project deliverables: i) newly synthesized pH- or H2O2-responsive polymers with controlled porosity, ii) pH- or H2O2-responsive polymers thin films with controlled hydrophobicity, porosity and thickness, iii) biosensors (enzyme-modified responsive polymer films) with tunable analytical figures of merit (limit of detection and linear dynamic range) according to the reference values of each biochemical index, iv) prototypes (hardware/software products), v) clinical and validation studies and vi) tools for the dissemination and exploitation of the results.