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BioPoc Technology

BioPoC Technology

The diagnostic devices employ novel responsive polymer-based biosensors and newly devised transduction and measuring principles. BioPoC devices include a single microfluidic vertical channel (Figure 1) and their operation is based on the measurement of the time required the infinite electric resistance between two, uncontacted, conductive layers to reach a finite value as a result of the selective degradation of the responsive polymer membrane by the target analyte (biochemical index). The concept is demonstrated by utilizing a pH-responsive membrane, a copolymer of methylmethacrylate and methacrylic acid (Eudragit S100), which degradated at pH>7, modified with the enzyme urease, for the drop volume (Figure 2) determination of urea in undiluted urine samples. In this case, membrane degradation is caused by the enzymatically produced ammonia according to the chemical equation: Urea + H2O –(urease) --> CO2 + 2NH3. The proposed diagnostic devices can be used as a generic platform for the point-of-care analysis of a large variety of biochemical indices, in accordance with the “ASSURED” criteria set by WHO. Wide scope applicability can be achieved by the proper combination of responsive polymer membranes and enzymes that will be immobilized onto the surface of the membranes. Medical diagnostic devices have been designed to have small size, light weight, and low power consumption.

Figure 1. Schematic representation and principle of operation of BioPoC biosensor
Figure 2. BioPoC prototype